Check out this USA patent application which is submitted by BSE epidimiologist John Collinge, and Charles Weissman of Great Britain. John Collinge is the primary epidemiologist that spread fear and lies about BSE, in the UK:
Quote:
United States Patent Application 20080108085
Kind Code A1
Enari; Masato; May 8, 2008
Colline, John; Weissmann, Charles et al.
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Method and Detection of the Presence of Prions Protein
Abstract
The invention relates to methods for determining the presence of prions in a tissue/organ or fluid therefrom; said method comprising the steps of: contacting the tissue/organ with one or more devices, wherein said devices are capable of binding prions; removing said devices from contact with said tissue/organ; determining if said devices are binding prions wherein the device is contacted with the tissue/organ for 120 minutes.
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Inventors: Enari; Masato; (Tokyo, JP) ; Flechsig; Eckhard; (Wurzburg, DE) ; Collinge; John; (London, GB) ; Weissmann; Charles; (London, GB)
jist of this application is that metal wires, preferrably wires smaller than 1 mm are capable of sequestering, or attaching to prions.
partial Quote:
Claims:
1. A method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: (a) contacting the tissue/organ with a device, wherein said device is capable of binding prions; and (b) removing said device from contact with said tissue/organ; and (c) determining if said device is binding prions.
2. A non-invasive method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: (a) contacting the tissue/organ with a device, wherein said device is capable of binding prions; (b) removing said device from contact with said tissue/organ; and (c) determining if said device is binding prions.
3. A method according to claim 1 wherein the device is capable of preserving prions against degradation.
4. A method according to claim 1 wherein the tissue/organ is a mammalian tissue/organ.
5. A method according to claim 4 wherein the tissue/organ is a livestock or a human tissue/organ.
6. A method according to claim 1 wherein the tissue/organ is an tissue/organ in which prions accumulate.
7. A method according to claim 6 wherein the tissue/organ is selected from brain, spleen, lymph node or tonsil.
8. A method according to claim 1 wherein the device comprises metal.
9. A method according to claim 8 wherein the metal comprises one or more metal(s) selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof.
..... some key parts in Description:
Quote:
[0004] Usually, diagnosis in humans relies on histopathology and immunohistochemical determination. Further methods for the diagnosis of prion infection require invasive procedures such as brain or tonsil biopsies. Homogenates of these biopsies are injected into the brains of test animals such as mice. If the test animals develop clinical symptoms of prion infection then the brain of the test animal is further examined to confirm that prions are present. Problems associated with this method are that prions contained within the biopsies are subject to degradation. Consequently, infectivity is usually lost within 24 hours.
...
[0006] The present invention provides methods for the detection of prions in a tissue/organ or fluid therefrom. The methods use a device such as a metal wire that is contacted with the tissue/organ. Surprisingly, the device is capable of binding prions within 5 minutes. The device is then removed from contact with the tissue/organ. Surprisingly, the device is able to preserve prions against degradation for greater than 3 days. Using prior art methods, prions degrade after only 24 hours. To determine if the device is binding prions, a number of different methods can be used as discussed below. Since prions bind to the device much faster than previously known, diagnosis of prion infection is significantly quicker than prior art methods.
[0007] According to the first aspect of the present invention, there is provided a method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: contacting the tissue/organ with a device, wherein said device is capable of binding prions; removing said device from contact with said tissue/organ; and determining if said device is binding prions.
[0008] According to a second aspect of the present invention, there is provided a non-invasive method for determining the presence of prions in a tissue/organ; said method comprising the steps of: contacting the tissue/organ with a device, wherein said device is capable of binding prions; removing said device from contact with said tissue/organ; and determining if said device is binding prions. Preferably, said intact tissue/organ is left at least substantially intact by said non-invasive method.
[0009] The device used in the methods of the present invention advantageously preserves prions against degradation.
NOTE: the device is a metal wire, in this patent application said to be steel, stainless steel, silver, gold or an alloy of these metals.
Quote:
[0012] The device of the present invention may comprise one or more metals or may comprise plastic such as polystyrene, or glass. It is surprisingly disclosed herein that these materials bind prion protein. Preferably, the device of the present invention may comprise one or more metals. Preferably, the metal is any one or more of the metals selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof. More preferably, the metal is stainless steel.
[0013] Advantageously, the device of the present invention may comprise one or more wires or spheres of diameter less than 5 mm, preferably less than 1 mm, preferably having dimensions as mentioned in the Examples section. Preferably, the device comprises one or more metal wires.
....
[0072] The binding between metals and prions may occur by any form of binding capable of occurring between metals and proteins such as covalent, ionic, Van Der Waals, transient or reversible association, or any other forms of binding interaction.
Preserving Prions
[0073] As used herein, the term "preserving prions" refers to the surprising finding disclosed in the present invention that when prions bind to metal surfaces they are preserved. As used herein, the term "preserved" means that the prions bound to the metal surface are protected against degradation and thus remain infective for a period of time that is longer than would normally be expected. For example, using prior art methods, prions injected into brain remain infective for about 24 hours only. Using the methods of the present invention, prions bound to a metal surface are advantageously preserved in barin for at least 3 days.
[0074] Advantageously, the device may be incubated at a temperature of about -20.degree. C. to further preserve the prions. The preservation may be further enhanced by any action which helps protect prions against degradation such as preventing prions from contacting proteases or preventing prions from contacting phagocytic cells.
[0079] Preferably, the device comprises one or more needles, spatula, pins, wires or spheres. More preferably, the device comprises one or more wires. Most preferably, the device comprises one or wires each measuring about 0.15 mm in diameter and 5 mm in length, such as stainless steel suture monofilament wire available from Braun MelsungerAG, Germany.
.....
[0291] Why are wire-bound prions as infectious as concentrated homogenates? Upon intracerebral inoculation with brain homogenate, infectivity is rapidly distributed throughout the mouse (18) and after 4 days or less prions are no longer detectable in the brain (19). Perhaps wire-bound prions are more stable and can therefore act over a longer period of time.
It is well known that the scrapie associated "fibril" is required for transmission of disease (experimental case studies)....
Therefore, this patent application by these UK prion "experts" demonstrates that the "partially malformed protein" will readily degrade in the phagocytic cells and within 24 hours, UNLESS the mechanisms to degrade them are depleted or gone, or UNLESS the partially misfolded proteins attach to metal spheres or wires that are "preferably less than 1mm".
That's a knock-out punch for Ms. Leuren Moret, and for Dr. Vitaly Vodyanoy.
Clearly, the disturbance of the brain by the metal wires/implants resulted in an upregulation of PrPC to protect the cells from the metal ions shedding from the wire. This up-regulation at the site of the wire resulted in the prion binding to the stainless steel - as the METALS in the wires were oxidized / ionized.
Thought I'd let you know about some of the mice that are tettering on the edge of the ships bridge - getting ready to jump ship.
This USA patent application clearly demonstrates the capability of metal wires or METAL SPHERES - just like depleted uranium (or DU alloys), at being able to nucleate proteins, in this case PrPC before the copper is adequately attached at all its normal binding locals.
Let's see the experiments with the "more dangerous" metals.
Quote:
United States Patent Application 20080108085
Kind Code A1
Enari; Masato; May 8, 2008
Colline, John; Weissmann, Charles et al.
--------------------------------------------------------------------------------
Method and Detection of the Presence of Prions Protein
Abstract
The invention relates to methods for determining the presence of prions in a tissue/organ or fluid therefrom; said method comprising the steps of: contacting the tissue/organ with one or more devices, wherein said devices are capable of binding prions; removing said devices from contact with said tissue/organ; determining if said devices are binding prions wherein the device is contacted with the tissue/organ for 120 minutes.
--------------------------------------------------------------------------------
Inventors: Enari; Masato; (Tokyo, JP) ; Flechsig; Eckhard; (Wurzburg, DE) ; Collinge; John; (London, GB) ; Weissmann; Charles; (London, GB)
jist of this application is that metal wires, preferrably wires smaller than 1 mm are capable of sequestering, or attaching to prions.
partial Quote:
Claims:
1. A method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: (a) contacting the tissue/organ with a device, wherein said device is capable of binding prions; and (b) removing said device from contact with said tissue/organ; and (c) determining if said device is binding prions.
2. A non-invasive method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: (a) contacting the tissue/organ with a device, wherein said device is capable of binding prions; (b) removing said device from contact with said tissue/organ; and (c) determining if said device is binding prions.
3. A method according to claim 1 wherein the device is capable of preserving prions against degradation.
4. A method according to claim 1 wherein the tissue/organ is a mammalian tissue/organ.
5. A method according to claim 4 wherein the tissue/organ is a livestock or a human tissue/organ.
6. A method according to claim 1 wherein the tissue/organ is an tissue/organ in which prions accumulate.
7. A method according to claim 6 wherein the tissue/organ is selected from brain, spleen, lymph node or tonsil.
8. A method according to claim 1 wherein the device comprises metal.
9. A method according to claim 8 wherein the metal comprises one or more metal(s) selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof.
..... some key parts in Description:
Quote:
[0004] Usually, diagnosis in humans relies on histopathology and immunohistochemical determination. Further methods for the diagnosis of prion infection require invasive procedures such as brain or tonsil biopsies. Homogenates of these biopsies are injected into the brains of test animals such as mice. If the test animals develop clinical symptoms of prion infection then the brain of the test animal is further examined to confirm that prions are present. Problems associated with this method are that prions contained within the biopsies are subject to degradation. Consequently, infectivity is usually lost within 24 hours.
...
[0006] The present invention provides methods for the detection of prions in a tissue/organ or fluid therefrom. The methods use a device such as a metal wire that is contacted with the tissue/organ. Surprisingly, the device is capable of binding prions within 5 minutes. The device is then removed from contact with the tissue/organ. Surprisingly, the device is able to preserve prions against degradation for greater than 3 days. Using prior art methods, prions degrade after only 24 hours. To determine if the device is binding prions, a number of different methods can be used as discussed below. Since prions bind to the device much faster than previously known, diagnosis of prion infection is significantly quicker than prior art methods.
[0007] According to the first aspect of the present invention, there is provided a method for detecting the presence of prions in a tissue/organ; said method comprising the steps of: contacting the tissue/organ with a device, wherein said device is capable of binding prions; removing said device from contact with said tissue/organ; and determining if said device is binding prions.
[0008] According to a second aspect of the present invention, there is provided a non-invasive method for determining the presence of prions in a tissue/organ; said method comprising the steps of: contacting the tissue/organ with a device, wherein said device is capable of binding prions; removing said device from contact with said tissue/organ; and determining if said device is binding prions. Preferably, said intact tissue/organ is left at least substantially intact by said non-invasive method.
[0009] The device used in the methods of the present invention advantageously preserves prions against degradation.
NOTE: the device is a metal wire, in this patent application said to be steel, stainless steel, silver, gold or an alloy of these metals.
Quote:
[0012] The device of the present invention may comprise one or more metals or may comprise plastic such as polystyrene, or glass. It is surprisingly disclosed herein that these materials bind prion protein. Preferably, the device of the present invention may comprise one or more metals. Preferably, the metal is any one or more of the metals selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof. More preferably, the metal is stainless steel.
[0013] Advantageously, the device of the present invention may comprise one or more wires or spheres of diameter less than 5 mm, preferably less than 1 mm, preferably having dimensions as mentioned in the Examples section. Preferably, the device comprises one or more metal wires.
....
[0072] The binding between metals and prions may occur by any form of binding capable of occurring between metals and proteins such as covalent, ionic, Van Der Waals, transient or reversible association, or any other forms of binding interaction.
Preserving Prions
[0073] As used herein, the term "preserving prions" refers to the surprising finding disclosed in the present invention that when prions bind to metal surfaces they are preserved. As used herein, the term "preserved" means that the prions bound to the metal surface are protected against degradation and thus remain infective for a period of time that is longer than would normally be expected. For example, using prior art methods, prions injected into brain remain infective for about 24 hours only. Using the methods of the present invention, prions bound to a metal surface are advantageously preserved in barin for at least 3 days.
[0074] Advantageously, the device may be incubated at a temperature of about -20.degree. C. to further preserve the prions. The preservation may be further enhanced by any action which helps protect prions against degradation such as preventing prions from contacting proteases or preventing prions from contacting phagocytic cells.
[0079] Preferably, the device comprises one or more needles, spatula, pins, wires or spheres. More preferably, the device comprises one or more wires. Most preferably, the device comprises one or wires each measuring about 0.15 mm in diameter and 5 mm in length, such as stainless steel suture monofilament wire available from Braun MelsungerAG, Germany.
.....
[0291] Why are wire-bound prions as infectious as concentrated homogenates? Upon intracerebral inoculation with brain homogenate, infectivity is rapidly distributed throughout the mouse (18) and after 4 days or less prions are no longer detectable in the brain (19). Perhaps wire-bound prions are more stable and can therefore act over a longer period of time.
It is well known that the scrapie associated "fibril" is required for transmission of disease (experimental case studies)....
Therefore, this patent application by these UK prion "experts" demonstrates that the "partially malformed protein" will readily degrade in the phagocytic cells and within 24 hours, UNLESS the mechanisms to degrade them are depleted or gone, or UNLESS the partially misfolded proteins attach to metal spheres or wires that are "preferably less than 1mm".
That's a knock-out punch for Ms. Leuren Moret, and for Dr. Vitaly Vodyanoy.
Clearly, the disturbance of the brain by the metal wires/implants resulted in an upregulation of PrPC to protect the cells from the metal ions shedding from the wire. This up-regulation at the site of the wire resulted in the prion binding to the stainless steel - as the METALS in the wires were oxidized / ionized.
Thought I'd let you know about some of the mice that are tettering on the edge of the ships bridge - getting ready to jump ship.
This USA patent application clearly demonstrates the capability of metal wires or METAL SPHERES - just like depleted uranium (or DU alloys), at being able to nucleate proteins, in this case PrPC before the copper is adequately attached at all its normal binding locals.
Let's see the experiments with the "more dangerous" metals.
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