WD9, Did you see this?
Abstract Sclerotinia sclerotiorum causes a highly destructive disease in oilseed **** (Brassica napus). Oxalic acid (OA) secreted by the pathogen is a key pathogenicity factor. Oxalate oxidase (OXO) can oxidize OA into CO2 and H2O2. In this study, we show that transgenic oilseed **** (sixth generation lines) constitutively expressing wheat (Triticum aestivum) OXO displays considerably increased OXO activity and enhanced resistance to S. sclerotiorum (with up to 90.2 and 88.4% disease reductions compared with the untransformed parent line and a resistant control, respectively). Upon application of exogenous OA, the pH values in transgenic plants were maintained at levels slightly lower than 5.58 measured prior to OA treatment, whereas the pH values in untransformed plants decreased rapidly and were markedly lower than 5.63 measured prior to OA treatment. Following pathogen inoculation, H2O2 levels were higher in transgenic plants than in untransformed plants. These results indicate that the enhanced resistance of the OXO transgenic oilseed **** to Sclerotinia is probably mediated by OA detoxification. We believe that enhancing the OA metabolism of oilseed **** in this way will be an effective strategy for improving resistance to S. sclerotiorum.
Xiangbai Dong1, 2, 3, Ruiqin Ji1, 2, 7, Xuelan Guo1, 2, Simon J. Foster4, Hong Chen5, Caihua Dong1, 2, Yueying Liu1, 2, Qiong Hu6 and Shengyi Liu1, 2
(1) The Key Laboratory of Genetic Improvement for Oil Crops, The Ministry of Agriculture, Wuhan, People’s Republic of China
(2) Department of Functional Genomics and Molecular Biology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences (OCRI-CAAS), Wuhan, 430062, People’s Republic of China
(3) Institute of Botany, Chinese Academy of Sciences, Beijing, Xiangshan, 100093, People’s Republic of China
(4) The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, UK
(5) Department of Analytic Chemistry and Food Quality, OCRI-CAAS, Wuhan, 430062, People’s Republic of China
(6) Department of Genetics and Breeding, OCRI-CAAS, Wuhan, 430062, People’s Republic of China
(7) Present address: College of Horticultural Sciences, Shenyang Agricultural University, Shenyang, 110161, People’s Republic of China
Received: 23 October 2007 Accepted: 26 March 2008 Published online: 30 April 2008
Keywords Hydrogen peroxide - Oilseed **** - Oxalic acid - Oxalate oxidase - Resistance - Sclerotinia
Xiangbai Dong and Ruiqin Ji contributed equally to this paper.
http://www.springerlink.com/content/85k3020164umn247/?p=f28c8e3cbfc34d5bb249e77acf714885&pi=0
Abstract Sclerotinia sclerotiorum causes a highly destructive disease in oilseed **** (Brassica napus). Oxalic acid (OA) secreted by the pathogen is a key pathogenicity factor. Oxalate oxidase (OXO) can oxidize OA into CO2 and H2O2. In this study, we show that transgenic oilseed **** (sixth generation lines) constitutively expressing wheat (Triticum aestivum) OXO displays considerably increased OXO activity and enhanced resistance to S. sclerotiorum (with up to 90.2 and 88.4% disease reductions compared with the untransformed parent line and a resistant control, respectively). Upon application of exogenous OA, the pH values in transgenic plants were maintained at levels slightly lower than 5.58 measured prior to OA treatment, whereas the pH values in untransformed plants decreased rapidly and were markedly lower than 5.63 measured prior to OA treatment. Following pathogen inoculation, H2O2 levels were higher in transgenic plants than in untransformed plants. These results indicate that the enhanced resistance of the OXO transgenic oilseed **** to Sclerotinia is probably mediated by OA detoxification. We believe that enhancing the OA metabolism of oilseed **** in this way will be an effective strategy for improving resistance to S. sclerotiorum.
Xiangbai Dong1, 2, 3, Ruiqin Ji1, 2, 7, Xuelan Guo1, 2, Simon J. Foster4, Hong Chen5, Caihua Dong1, 2, Yueying Liu1, 2, Qiong Hu6 and Shengyi Liu1, 2
(1) The Key Laboratory of Genetic Improvement for Oil Crops, The Ministry of Agriculture, Wuhan, People’s Republic of China
(2) Department of Functional Genomics and Molecular Biology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences (OCRI-CAAS), Wuhan, 430062, People’s Republic of China
(3) Institute of Botany, Chinese Academy of Sciences, Beijing, Xiangshan, 100093, People’s Republic of China
(4) The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, UK
(5) Department of Analytic Chemistry and Food Quality, OCRI-CAAS, Wuhan, 430062, People’s Republic of China
(6) Department of Genetics and Breeding, OCRI-CAAS, Wuhan, 430062, People’s Republic of China
(7) Present address: College of Horticultural Sciences, Shenyang Agricultural University, Shenyang, 110161, People’s Republic of China
Received: 23 October 2007 Accepted: 26 March 2008 Published online: 30 April 2008
Keywords Hydrogen peroxide - Oilseed **** - Oxalic acid - Oxalate oxidase - Resistance - Sclerotinia
Xiangbai Dong and Ruiqin Ji contributed equally to this paper.
http://www.springerlink.com/content/85k3020164umn247/?p=f28c8e3cbfc34d5bb249e77acf714885&pi=0
Comment