What's an HPLC?
Announcement
Collapse
No announcement yet.
Residue Tolerances for Malathion Sharply Reduced
Collapse
Logging in...
Welcome to Agriville! You need to login to post messages in the Agriville chat forums. Please login below.
X
-
High Performance Liquid Chromatography aka H Pressure LC. The basic uses, chemical seperation, purification, identification, and quantification. Sorry you asked yet?
Basic use is determine the concentration of a chemical compound (malathion) in a known chemical compound (wheat).
There are three types of chromatographs, gas (which most people have heard of) liquid, and supercritical. Chroma means light, specifically color and graph, well, means to graph
The liquid gets its name from disolving the compound in a solvent like water or alcohol, shoot the stuff thru a tube filled with silicon beads at high pressure and use light and detectors to measure the chemicals (malathion) as they pass thru at different speeds to determine concentration in the known main compound (wheat).
Not exactly how it works, highly simplified, but close enough. I hope Dr. Jim Daun isn't reading this, sorry Jim in advance
Now you know.
Comment
-
I could also add that electrophoresis which is used to determine varieties of grain uses the technique that if you pass electricity thru a gel, add the mooshed up grain to the gel, the individual proteins will migrate at different rates thru the gel and end up looking like a bar code.
Each variety has its own "bar code" picture and is easily identifiable. The HPLC uses the same kind of technique except in the form of gasses moving at different speeds. Ok, I'll stop.
Comment
- Reply to this Thread
- Return to Topic List
Comment